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Billions of online video ads are viewed every month. We present a large-scale analysis of facial responses to video content measured over the Internet and their relationship to marketing effectiveness. We collected over 12,000 facial responses from 1,223 people to 170 ads from a range of markets and product categories. The facial responses were automatically coded frame-by-frame. Collection and coding of these 3.7 million frames would not have been feasible with traditional research methods. We show that detected expressions are sparse but that aggregate responses reveal rich emotion trajectories. By modeling the relationship between the facial responses and ad effectiveness, we show that ad liking can be predicted accurately (ROC AUC = 0.85) from webcam facial responses. Furthermore, the prediction of a change in purchase intent is possible (ROC AUC = 0.78). Ad liking is shown by eliciting …